Proteomics Surveillance of the Influenza Virus with Mass Spectrometry
Our laboratory pioneered (beginning 1999) the development and application of advanced mass spectrometry and proteomics approaches to characterise both the antigencity of the influenza virus, and also to assess the type, subtype and lineage of circulating strains directly from whole virus digests.
The combined approaches have advantages over present surveillance methods.
Relevant references on influenza :
Schwahn AB, Wong JWH, Downard KM (2009) Subtyping of the Influenza Virus by High Resolution Mass Spectrometry, Anal. Chem., 81, 3500–3506.
Schwahn AB, Downard KM (2009) Antigenicity of a type A influenza virus through comparison of hemagglutination inhibition and mass spectrometry immunoassays,J. Immunoassay Immunochem.. 30: 245-261.
Downard KM, Morrissey B, Schwahn AB (2009) Mass Spectrometry Analysis of the Influenza Virus, Mass Spectrom. Rev., 28: 35-49.
Downard KM, Morrissey B (2007) Fingerprinting a Killer - Surveillance of the Influenza Virus by
Mass Spectrometry, The Analyst, 132, 611–614.
Morrissey B, Streamer M, Downard KM (2007) Antigenic Characterisation of H3N2 subtypes of the Influenza Virus by Mass Spectrometry, J. Virol. Methods, 145: 106–114.
Morrissey B, Downard KM (2006) A Proteomics Approach to Survey the Antigenicity of the Influenza Virus by Mass Spectrometry, Proteomics, 6: 2034-2041.
Kiselar JG, Downard KM (1999) Antigenic Surveillance of the Influenza Virus by Mass Spectrometry, Biochemistry 43: 14185-14191.
Related references on immunoassay development:
Morrissey B, Downard KM (2008) Kinetics of Antigen-Antibody Interactions Employing a MALDI Mass Spectrometry Immunoassay, Anal. Chem., 80: 7720-7726.
Kiselar J.G., Downard K.M. (2000) Preservation and Detection of Specific Antibody-Peptide Complexes by Matrix-assisted Laser Desorption Ionization Mass Spectrometry, J. Am.Soc Mass Spectrom., 11: 746-750.
Kiselar JG, Downard KM (1999) Direct identification of protein epitopes by mass spectrometry without immobilization of antibody and isolation of antibody–peptide complexes, Anal. Chem., 71: 1792–1799.
