Associate Professor Kevin Downard
Associate Professor G08 - Biochemistry and Microbiology Building
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Our research interests focus on the study of protein interactions using cutting edge mass spectrometry and proteomics approaches that we both develop and apply.
Current research projects :
1. Viral proteomics using MALDI-MS for surveillance of the type, subtype and antigenicity of the influenza virus to monitor the evolution of the virus and identify the emergence of highly virulent new strains.
2. Application of Radical Probe Mass Spectrometry (RP-MS) to study protein interaction interfaces and the impact of oxidation on these interactions. Our research is focussed particularly on long lived tissue proteins.
3. Development and application of new computer algorithms (eg. COMPLX, PROXIMO and PRISM) and approaches to obtain information on the structure and interactions of proteins from mass spectra and to advance bioinformatics. Other algorithms to deconvolute ESI mass spectra (CRAM) and advance "shotgun" proteomics (ETISEQ) have also been written.
PhD research opportunities are available to qualified local and overseas students.
Scholarship application details can be found at: http://www.usyd.edu.au/ro/training/index.shtml
Current research projects :
1. Viral proteomics using MALDI-MS for surveillance of the type, subtype and antigenicity of the influenza virus to monitor the evolution of the virus and identify the emergence of highly virulent new strains.
2. Application of Radical Probe Mass Spectrometry (RP-MS) to study protein interaction interfaces and the impact of oxidation on these interactions. Our research is focussed particularly on long lived tissue proteins.
3. Development and application of new computer algorithms (eg. COMPLX, PROXIMO and PRISM) and approaches to obtain information on the structure and interactions of proteins from mass spectra and to advance bioinformatics. Other algorithms to deconvolute ESI mass spectra (CRAM) and advance "shotgun" proteomics (ETISEQ) have also been written.
PhD research opportunities are available to qualified local and overseas students.
Scholarship application details can be found at: http://www.usyd.edu.au/ro/training/index.shtml
- Downard, K. J. J. Thomson Goes to America. Journal of the American Society for Mass Spectrometry. 2009; 20:1964-73 [Abstract]
- Diemer, H, Atmanene, C, Sanglier, S, Morrissey, B, Van Dorsselaer, A, Downard, K. Detection and structural features of the betaB2-B3-crystallin heterodimer by radical probe mass spectrometry (RP-MS). Journal of mass spectrometry. 2009; 44:803-812 [Abstract]
- Schwahn, A, Wong, J, Downard, K. Subtyping of the influenza virus by high resolution mass spectrometry. Analytical chemistry. 2009; 81:3500-3506 [Abstract]
- Downard, K, Morrissey, B, Schwahn, A. Mass spectrometry analysis of the influenza virus. Mass spectrometry reviews. 2009; 28:35-49 [Abstract]
- Downard, K. Editorial: Australia contributors' issue. Mass spectrometry reviews. 2009; 28:1 [Abstract]
- Schwahn, A, Downard, K. Antigenicity of a type A influenza virus through comparison of hemagglutination inhibition and mass spectrometry immunoassays. Journal of immunoassay & immunochemistry. 2009; 30:245-261 [Abstract]
- Wong, J, Schwahn, A, Downard, K. ETISEQ--an algorithm for automated elution time ion sequencing of concurrently fragmented peptides for mass spectrometry-based proteomics. BMC bioinformatics. 2009; 10:244 [Abstract]
- Morrissey, B, Downard, K. Kinetics of antigen-antibody interactions employing a MALDI mass spectrometry immunoassay. Analytical chemistry. 2008; 80:7720-6 [Abstract]
- Morrissey, B, Streamer, M, Downard, K. Antigenic characterisation of H3N2 subtypes of the influenza virus by mass spectrometry. Journal of virological methods. 2007; 145:106-14 [Abstract]
- Downard, K, Morrissey, B. Fingerprinting a killer: surveillance of the influenza virus by mass spectrometry. The Analyst. 2007; 132:611-614 [Abstract]
- Ho, J, Morrissey, B, Downard, K. A Computer Algorithm for the Identification of Protein Interactions from the Spectra of Masses (PRISM). Journal of the American Society for Mass Spectrometry. 2007; 18:563-6 [Abstract]
- Maleknia, S, Downard, K. Genesis and Application of Radical Probe Mass Spectrometry (RP-MS) to Study Protein Interactions, in/ Mass Spectrometry of Protein Interactions. In: Mass Spectrometry of Protein Interactions. New Jersey, USA: John Wiley & Sons, Inc. 2007. p. 109-133.
- Downard, K. Softly softly - Dectection of protein complexes by matrix-assisted laser desorption ionization mass spectrometry. In: Mass Spectrometry of Protein Interactions. New Jersey, USA: John Wiley & Sons 2007. p. 25-43.
- Issa, S, Downard, K. Interaction between alpha and upsilon-crystallin, common to the eye of the Australian platypus, by radical probe mass spectrometry. Journal of mass spectrometry : JMS. 2006; 41:1298-303 [Abstract]
- Downard, K. Ions of the interactome: The role of MS in the study of protein interactions in proteomics and structural biology. Proteomics. 2006; 6:5374-84 [Abstract]
- Gerega, S, Downard, K. PROXIMO - a new algorithm to model protein complexes using data from Radical Probe Mass Spectrometry (RP-MS). Bioinformatics (Oxford, England). 2006; 22:1702-9 [Abstract]
- Morrissey, B, Downard, K. A proteomics approach to survey the antigenicity of the influenza virus by mass spectrometry. Proteomics. 2006; 6:2034-41 [Abstract]
- Downard, K. Mass Spectrometry - A Foundation Course. United Kingdom: Royal Society of Chemistry, 2004.
- Mackun, K, Downard, K. Strategy for identifying protein-protein interactions of gel-separated proteins and complexes by mass spectrometry. Analytical biochemistry. 2003; 318:60-70 [Abstract]
- Wong, J, Downard, K. COMPLX: a computer algorithm for the detection of protein-ligand and other macromolecular complexes in mass spectra. Journal of mass spectrometry. 2003; 38:573-581 [Abstract]
- Wong, J, Maleknia, S, Downard, K. Study of the ribonuclease-S-protein-peptide complex using a radical probe and electrospray ionization mass spectrometry. Analytical Chemistry. 2003; 75:1557-1563 [Abstract]
mass spectrometry; protein structure; protein interactions; proteomics; complexes; bioinformatics; computer algorithms; influenza virus; protein; protein microarray; MS; computer programming; protein complexes