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The University of Sydney
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Professor Philip William Kuchel

Professor and ARC Australian Professorial Fellow
Molecular and Microbial Biosciences
Faculty of Science

G08 - Biochemistry and Microbiology Building
The University of Sydney
NSW 2006 Australia

Email
Phone +61 2 9351 3709
Fax +61 2 9351 4726
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Research Interests

My group works in the general area of Physical Biochemistry. We study contemporary problems in the interface between biology, and the physical sciences. Much of our work has implications for the understanding of cellular function at the molecular level and hence is of relevance to the fundamental basis of disease.

Our major technological focus is NMR spectroscopy. We use this technique to study metabolic and membrane transport processes in cells, mostly the human erythrocyte. We also make extensive use of NMR to determine the structure in solution of peptides and proteins. Recent emphasis has been on the components of platypus venom and a potassium ion channel from heart. Our other emphasis is on mathematical modelling of biochemical systems, especially metabolism, and for this we make extensive use of Mathematica.

Select Publications

    2009
    • Peslova, G, Petrak, J, Kuzelova, K, Hrdy, I, Halada, P, Kuchel, P, Soe-Lin, S, Ponka, P, Sutak, R, Becker, E, Huang, M, Suryo Rahmanto, Y, Richardson, D, Vyoral, D. Hepcidin, the hormone of iron metabolism, is bound specifically to {alpha}-2-macroglobulin in blood. Blood. 2009; 113:6225-36 [Abstract]
    • Whittington, C, Koh, J, Warren, W, Papenfuss, A, Torres, A, Kuchel, P, Belov, K. Understanding and utilising mammalian venom via a platypus venom transcriptome. Journal of proteomics. 2009; 72:155-64 [Abstract]
    • Ju, P, Pages, G, Riek, R, Chen, P, Torres, A, Bansal, P, Kuyucak, S, Kuchel, P, Vandenberg, J. The pore domain outer helix contributes to both activation and inactivation of the hERG K+ channel. The Journal of biological chemistry. 2009; 284:1000-8 [Abstract]
    2008
    • Pages, G, Szekely, D, Kuchel, P. Erythrocyte-shape evolution recorded with fast-measurement NMR diffusion-diffraction. Journal of magnetic resonance imaging : JMRI. 2008; 28:1409-16 [Abstract]
    • Perrin, M, Kuchel, P, Campbell, T, Vandenberg, J. Drug binding to the inactivated state is necessary but not sufficient for high affinity binding to hERG channels. Molecular pharmacology. 2008; 74:1443-52 [Abstract]
    • Naumann, C, Kuchel, P. Prochiral and chiral resolution in 2H NMR spectra: solutes in stretched and compressed gelatin gels. The journal of physical chemistry. A. 2008; 112:8659-64 [Abstract]
    • Whittington, C, Papenfuss, A, Kuchel, P, Belov, K. Expression patterns of platypus defensin and related venom genes across a range of tissue types reveal the possibility of broader functions for OvDLPs than previously suspected. Toxicon : official journal of the International Society on Toxinology. 2008; 52:559-65 [Abstract]
    • Torres, A, Bubb, W, Philp, D, Kuchel, P. Improved J-compensated sequences based on short composite pulses. Journal of magnetic resonance (San Diego, Calif. : 1997). 2008; 194:81-8 [Abstract]
    • Kuchel, P, Philp, D. Isotopomer subspaces as indicators of metabolic-pathway structure. Journal of theoretical biology. 2008; 252:391-401 [Abstract]
    • Whittington, C, Papenfuss, A, Bansal, P, Torres, A, Wong, E, Deakin, J, Graves, T, Alsop, A, Schatzkamer, K, Kremitzki, C, Ponting, C, Temple-Smith, P, Warren, W, Kuchel, P, Belov, K. Defensins and the convergent evolution of platypus and reptile venom genes. Genome research. 2008; 18:986-94 [Abstract]
    • Momot, K, Takegoshi, K, Kuchel, P, Larkin, T. Inhomogeneous NMR Line Shape as a Probe of Microscopic Organization of Bicontinuous Cubic Phases. The journal of physical chemistry. B. 2008; 112:6636-6645 [Abstract]
    • Kuchel, P, Naumann, C. 2H2O quadrupolar splitting used to measure water exchange in erythrocytes. Journal of magnetic resonance (San Diego, Calif. : 1997). 2008; 192:48-59 [Abstract]
    • Bansal, P, Torres, A, Crossett, B, Wong, K, Koh, J, Geraghty, D, Vandenberg, J, Kuchel, P. Substrate specificity of platypus venom L-to-D-peptide isomerase. The Journal of biological chemistry. 2008; 283:8969-75 [Abstract]
    • Larkin, T, Bubb, W, Kuchel, P. Water chemical shift in 1H NMR of red cells: effects of pH when transmembrane magnetic susceptibility differences are low. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. 2008; 59:707-11 [Abstract]
    • Ryan, D, Duncan, J, Lee, C, Kuchel, P, Matthews, J. Assembly of the oncogenic DNA-binding complex LMO2-Ldb1-TAL1-E12.Assembly of the oncogenic DNA-binding complex LMO2-Ldb1-TAL1-E12. Proteins. 2008; 70:1461-74 [Abstract]
    • Kuchel, P. 10n2 + 2 revealed. Mathematical Gazette. 2008; 92:546-551
    • Whillier, S, Raftos, J, Kuchel, P. Glutathione synthesis by red blood cells in type 2 diabetes mellitus. Redox Report. 2008; 13:277-282 [Abstract]
    2007
    • Plummer, R, Bodkin, J, Power, D, Pantarat, N, Bubb, W, Kuchel, P, Sorrell, T. Effect of Caspofungin on Metabolite Profiles of Aspergillus Species Determined by Nuclear Magnetic Resonance Spectroscopy. Antimicrobial agents and chemotherapy. 2007; 51:4077-84 [Abstract]
    • Plummer, R, Bodkin, J, Yau, T, Power, D, Pantarat, N, Larkin, T, Szekely, D, Bubb, W, Sorrell, T, Kuchel, P. Modelling Staphylococcus aureus-induced septicemia using NMR. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. 2007; 58:656-665 [Abstract]
    • Hill, A, Campbell, T, Bansal, P, Kuchel, P, Vandenberg, J. The S631A Mutation Causes a Mechanistic Switch in the Block of hERG Channels by CnErg1. Biophysical journal. 2007; 93:L32-L34 [Abstract]
    • Naumann, C, Bubb, W, Chapman, B, Kuchel, P. Tunable-alignment chiral system based on gelatin for NMR spectroscopy. Journal of the American Chemical Society. 2007; 129:5340-5341 [Abstract]
    • Larkin, T, Bubb, W, Kuchel, P. pH and cell volume effects on H2O and phosphoryl resonance splitting in rapid-spinning NMR of red cells. Biophysical journal. 2007; 92:1770-6 [Abstract]
    • Raftos, J, Kuchel, P, Whillier, S, Chapman, B. Kinetics of uptake and deacetylation of N-acetylcysteine by human erythrocytes. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY. 2007; 39:1698-1706 [Abstract]
    • Torres, A, Tsampazi, M, Kennett, E, Belov, K, Geraghty, D, Bansal, P, Alewood, P, Kuchel, P. Characterization and isolation of L: -to-D: -amino-acid-residue isomerase from platypus venom. Amino acids. 2007; 32:63-8 [Abstract]
    • Kuchel, P. Can you 'bend' a truncated octahedron?. Mathematical Gazette. 2007; 91:533-536
    • Ju, P, Torres, A, Bansal, P, Kuchel, P, Vandenberg, J. Pore helix domain charged residues modulate inactivation of the hERG K+ channel. BIOPHYSICAL JOURNAL. 2007; 0:123A-123A [Abstract]
    2006
    • Coen, M, Bodkin, J, Power, D, Bubb, W, Himmelreich, U, Kuchel, P, Sorrell, T. Antifungal effects on metabolite profiles of medically important yeast species measured by nuclear magnetic resonance spectroscopy. Antimicrobial agents and chemotherapy. 2006; 50:4018-26 [Abstract]
    • Szekely, D, Chapman, B, Bubb, W, Kuchel, P. Rapid Exchange of Fluoroethylamine via the Rhesus Complex in Human Erythrocytes: (19)F NMR Magnetization Transfer Analysis Showing Competition by Ammonia and Ammonia Analogues. Biochemistry. 2006; 45:9354-61 [Abstract]
    • Momot, K, Kuchel, P. PFG NMR diffusion experiments for complex systems. Concepts in Magnetic Resonance Part A. 2006; 28A:249-269 [Abstract]
    • Kuchel, P, Chapman, B, Müller, N, Bubb, W, Philp, D, Torres, A. Apparatus for rapid adjustment of the degree of alignment of NMR samples in aqueous media: verification with residual quadrupolar splittings in (23)Na and (133)Cs spectra. Journal of magnetic resonance (San Diego, Calif. : 1997). 2006; 180:256-65 [Abstract]
    • Maher, A, Chapman, B, Kuchel, P. (39)K nuclear magnetic resonance and a mathematical model of K(+) transport in human erythrocytes. European biophysics journal : EBJ. 2006; 35:293-301 [Abstract]
    • Torres, A, Tsampazi, M, Tsampazi, C, Kennett, E, Belov, K, Geraghty, D, Bansal, P, Alewood, P, Kuchel, P. Mammalian l-to-d-amino-acid-residue isomerase from platypus venom. FEBS letters. 2006; 580:1587-91 [Abstract]
    • Beves, J, Chapman, B, Kuchel, P, Lindoy, L, McMurtrie, J, McPartlin, M, Thordarson, P, Wei, G. New discrete metallocycles incorporating palladium(II) and platinum(II) corners and dipyridyldibenzotetraaza[14]annulene side units. Dalton transactions (Cambridge, England : 2003). 2006; 2006:744-750 [Abstract]
    • Kennett, E, Kuchel, P. Plasma membrane oxidoreductases: effects on erythrocyte metabolism and redox homeostasis. Antioxidants & redox signaling. 2006; 8:1241-7 [Abstract]
    • de Atauri, P, Ramirez, M, Kuchel, P, Carreras, J, Cascante, M. Metabolic homeostasis in the human erythrocyte: In silico analysis. Biosystems. 2006; 83:118-124 [Abstract]
    2005
    • Coen, M, O'Sullivan, M, Bubb, W, Kuchel, P, Sorrell, T. Proton nuclear magnetic resonance-based metabonomics for rapid diagnosis of meningitis and ventriculitis. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America. 2005; 41:1582-90 [Abstract]
    • Kuchel, P, Benga, G. Why does the mammalian red blood cell have aquaporins?. Bio Systems. 2005; 82:189-96 [Abstract]
    • Torres, A, Tsampazi, C, Geraghty, D, Bansal, P, Alewood, P, Kuchel, P. D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties. The Biochemical journal. 2005; 391:215-20 [Abstract]
    • Momot, K, Kuchel, P, Chapman, B. Acquisition of pure-phase diffusion spectra using oscillating-gradient spin echo. Journal of magnetic resonance (San Diego, Calif.: 1997). 2005; 176:151-9 [Abstract]
    • Selivanov, V, Meshalkina, L, Solovjeva, O, Kuchel, P, Ramos-Montoya, A, Kochetov, G, Lee, P, Cascante, M. Rapid simulation and analysis of isotopomer distributions using constraints based on enzyme mechanisms: an example from HT29 cancer cells. Bioinformatics (Oxford, England). 2005; 21:3558-64 [Abstract]
    • Kennett, E, Ogawa, E, Agar, N, Godwin, I, Bubb, W, Kuchel, P. Investigation of methaemoglobin reduction by extracellular NADH in mammalian erythrocytes. The international journal of biochemistry & cell biology. 2005; 37:1438-45 [Abstract]
    • Nouri-Sorkhabi, M, Chapman, B, O'Loughlin, E, Li, Z, Kuchel, P, Gaskin, K. NMR measurements of the diffusional permeability of water in cultured colonic epithelial cancer cells. Cell biology international. 2005; 29:441-8 [Abstract]
    • Momot, K, Kuchel, P. Convection-compensating diffusion experiments with phase-sensitive double-quantum filtering. Journal of magnetic resonance (San Diego, Calif.: 1997). 2005; 174:229-36 [Abstract]
    • Torres, A, Philp, D, Kemp-Harper, R, Garvey, C, Kuchel, P. Determination of Na+ binding parameters by relaxation analysis of selected Na-23 NMR coherences: RNA, BSA and SDS. Magnetic Resonance in Chemistry. 2005; 43:217-224 [Abstract]
    • Philp, D, Kuchel, P. A way of visualizing NMR experiments on quadrupolar nuclei. Concepts in Magnetic Resonance Part A. 2005; 25A:40-52 [Abstract]
    • Kuchel, P, Prentice, K, Percival, F. O'Leary's precision timekeeper. Horological Journal. 2005; Jan:20-24
    • Kuchel, P. Making a chain from a solid bar. Horological Journal. 2005; 147:106-107
    • Kennett, E, Bubb, W, Bansal, P, Alewood, P, Kuchel, P. NMR studies of exchange between intra- and extracellular glutathione in human erythrocytes. Redox report: communications in free radical research. 2005; 10:83-90 [Abstract]
    2004
    • Kuchel, P, Bubb, W, Ramadan, S, Chapman, B, Philp, D, Coen, M, Gready, J, Harvey, P, McLean, A, Hook, J. 31P MAS-NMR of human erythrocytes: independence of cell volume from angular velocity. Magnetic Resonance in Medicine. 2004; 52:663-668 [Abstract]
    2003
    • Benga, G, Chapman, B, Cox, G, Kuchel, P. Comparative NMR studies of diffusional water permeability of red blood cells from different species: XIV. Little Penguin, Eudyptula minor. Cell biology international. 2003; 27:921-8 [Abstract]
    2002
    • Torres, A, Bubb, W, Kuchel, P. Experiments to detect long-range heteronuclear shift correlations: LR-J-HSMQC. Journal of magnetic resonance (San Diego, Calif. : 1997). 2002; 156:249-57 [Abstract]
    • Bourne, R, Kuchel, P. Device for aeration and mixing of cell and organelle suspensions during NMR experiments. Journal Of Magnetic Resonance. 2002; 159:158-160
    • Rohwer, J, Kuchel, P, Maher, A. ThermoKinetic modelling. Membrane potential as a dependent variable in ion transport processes. Molecular biology reports. 2002; 29:217-25 [Abstract]
    2001
    • Toonkool, P, Regan, D, Kuchel, P, Morris, M, Weiss, A. Thermodynamic and hydrodynamic properties of human tropoelastin. Analytical ultracentrifuge and pulsed field-gradient spin-echo NMR studies. The Journal of biological chemistry. 2001; 276:28042-50 [Abstract]
    • Nouri-Sorkhabi, M, Chapman, B, Kuchel, P, O'Loughlin, E, Gaskin, K. Water Transport in epithelial cells expressing CFTR. Pediatric Pulmonology. 2001; 88:255-258
    • Gaskin, K, Nouri-Sorkhabi, M, Gruca, M, Kuchel, P. Intestinal phospholipid metabolism: Its influence on fat digestion and absorption. Pediatric Pulmonology. 2001; 1 (5):260-266
    • Hardie, K, Nouri-Sorkhabi, M, Nouri-Sorkhabi, M, Freedman, S, Kuchel, P, Gaskin, K. Elevated pancreatic GP-2 secretion in pancreatic sufficient (PS) CF patients. Pediatric Pulmonology. 2001; 44:1118-1120
    2000
    • Himmelreich, U, Emling, M, Drew, K, Serianni, A, Kuchel, P. 13C NMR evidence of the failure of human erythrocytes to metabolise ascorbate and dehydroascorbate to lactate. Free Radical Biology and Medicine. 2000; 28:1607-1610
    • Hossein Nouri-Sorkhabi, M, Chapman, B, Kuchel, P, Gruca, M, Gaskin, K. Parallel secretion of pancreatic phosopholipase A2, phospholipase A1, lipase, and colipase in children with exocrine pancreatic dysfunction. Pediatric Research. 2000; 48:735-740

    Publication Keywords

    Erythrocytes; Magnetic Resonance Spectroscopy; Pentose Phosphate Pathway; Mathematical models of metabolism; Isotopomer analysis; NMR diffusion measurements; Diffusion diffraction; Platypus venom; Haemoglobin; Defensin-like peptides (DLPs); Water transport; Mathematica; Glycolysis; Glutathione metabolism

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