School of Molecular and Microbial Biosciences
The University of Sydney
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Professor Anthony Weiss

Professor
Molecular and Microbial Biosciences
Faculty of Science

G08 - Biochemistry and Microbiology Building
The University of Sydney
NSW 2006 Australia

Email
Phone +61 2 9351 3464
Fax +61 2 9351 4726
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Research Interests

The Weiss Lab's research focuses on the assembly of human elastic tissue, damage and its repair. We are very interested in the amazing, self-assembling elastic protein tropoelastin and the use of synthetic elastin to repair elastic tissues in skin, artery, bladder and lung.

Select Publications

    2009
    • Bax, D, McKenzie, D, Weiss, A, Bilek, M. Linker-free covalent attachment of the extracellular matrix protein tropoelastin to a polymer surface for directed cell spreading. Acta biomaterialia. 2009; 5:3371-81 [Abstract]
    • Bax, D, Rodgers, U, Bilek, M, Weiss, A. Cell adhesion to tropoelastin is mediated via the C-terminal GRKRK motif and integrin alphaVbeta3. The Journal of biological chemistry. 2009; 284:28616-23 [Abstract]
    • Annabi, N, Mithieux, S, Boughton, E, Ruys, A, Weiss, A, Dehghani, F. Synthesis of highly porous crosslinked elastin hydrogels and their interaction with fibroblasts in vitro. Biomaterials. 2009; 30:4550-7 [Abstract]
    • Dyksterhuis, L, Carter, E, Mithieux, S, Weiss, A. Tropoelastin as a thermodynamically unfolded premolten globule protein: The effect of trimethylamine N-oxide on structure and coacervation. Archives of biochemistry and biophysics. 2009; 487:79-84 [Abstract]
    • Naumann, C, Mithieux, S, Szekely, D, Tu, Y, Weiss, A, Kuchel, P. "Setting paint" analogy for the hydrophobic self-association of tropoelastin into elastin-like hydrogel. Biopolymers. 2009; 91:321-330 [Abstract]
    • Wise, S, Weiss, A. Tropoelastin. The international journal of biochemistry & cell biology. 2009; 41:494-7 [Abstract]
    • Yin, Y, Wise, S, Nosworthy, N, Waterhouse, A, Bax, D, Youssef, H, Byrom, M, Bilek, M, McKenzie, D, Weiss, A, Ng, M. Covalent immobilisation of tropoelastin on a plasma deposited interface for enhancement of endothelialisation on metal surfaces. Biomaterials. 2009; 30:1675-81 [Abstract]
    • Mithieux, S, Tu, Y, Korkmaz, E, Braet, F, Weiss, A. In situ polymerization of tropoelastin in the absence of chemical cross-linking. Biomaterials. 2009; 30:431-5 [Abstract]
    • Annabi, N, Mithieux, S, Weiss, A, Dehghani, F. The fabrication of elastin-based hydrogels using high pressure CO(2). Biomaterials. 2009; 30:1-7 [Abstract]
    2008
    • Tu, Y, Weiss, A. Glycosaminoglycan-mediated coacervation of tropoelastin abolishes the critical concentration, accelerates coacervate formation, and facilitates spherule fusion: implications for tropoelastin microassembly. Biomacromolecules. 2008; 9:1739-44 [Abstract]
    • Macdonald, C, Morrow, R, Weiss, A, Bilek, M. Covalent attachment of functional protein to polymer surfaces: a novel one-step dry process. Journal of the Royal Society, Interface / the Royal Society. 2008; 5:663-9 [Abstract]
    • Taddese, S, Weiss, A, Neubert, R, Schmelzer, C. Mapping of macrophage elastase cleavage sites in insoluble human skin elastin. Matrix biology : journal of the International Society for Matrix Biology. 2008; 27:420-8 [Abstract]
    • Dehghani, F, Annabi, N, Valtchev, P, Mithieux, S, Weiss, A, Kazarian, S, Tay, F. Effect of dense gas CO2 on the coacervation of elastin. Biomacromolecules. 2008; 9:1100-1105 [Abstract]
    2007
    • Muiznieks, L, Weiss, A. Flexibility in the solution structure of human tropoelastin. Biochemistry. 2007; 46:8196-8205 [Abstract]
    • Dyksterhuis, L, Baldock, C, Lammie, D, Wess, T, Weiss, A. Domains 17-27 of tropoelastin contain key regions of contact for coacervation and contain an unusual turn-containing crosslinking domain. Matrix biology : journal of the International Society for Matrix Biology. 2007; 26:125-35 [Abstract]
    • Clarke, A, Foster, T, Keane, F, Weiss, A. The N-terminal a domain of Staphylococcus aureus fibronectin-binding protein a binds to tropoelastin. BIOCHEMISTRY. 2007; 46:7226-7232 [Abstract]
    2006
    • Nicoloff, G, Weiss, A, Iotova, V, Tzaneva, V, Petrova, C, Domuschieva, N, Nikolov, A, Tzvetanov, P, Christova, P. Abnormal levels of serum antielastin antibodies in children with diabetes mellitus type 1. Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 2006; 54:461-7 [Abstract]
    • Riley, L, Roufogalis, B, Li, G, Weiss, A. A radioassay for synaptic core complex assembly: Screening of herbal extracts for effectors. Analytical biochemistry. 2006; 357:50-7 [Abstract]
    • Clarke, A, Arnspang, E, Mithieux, S, Korkmaz, E, Braet, F, Weiss, A. Tropoelastin Massively Associates during Coacervation To Form Quantized Protein Spheres. Biochemistry. 2006; 45:9989-9996 [Abstract]
    • Lemire, J, Patis, C, Gordon, L, Sandy, J, Toole, B, Weiss, A. Aggrecan expression is substantially and abnormally upregulated in Hutchinson-Gilford Progeria Syndrome dermal fibroblasts. Mechanisms of ageing and development. 2006; 127:660-9 [Abstract]
    2005
    • Li, M, Mondrinos, M, Gandhi, M, Ko, F, Weiss, A, Lelkes, P. Electrospun protein fibers as matrices for tissue engineering. Biomaterials. 2005; 26:5999-6008 [Abstract]
    • Rodgers, U, Weiss, A. Cellular interactions with elastin. Pathologie-biologie. 2005; 53:390-8 [Abstract]
    • Cain, S, Baldock, C, Gallagher, J, Morgan, A, Bax, D, Weiss, A, Shuttleworth, C, Kielty, C. Fibrillin-1 interactions with heparin - Implications for microfibril and elastic fiber assembly. Journal of Biological Chemistry. 2005; 280:30526-30537 [Abstract]
    • Clarke, A, Wise, S, Cain, S, Kielty, C, Weiss, A. Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin. Biochemistry. 2005; 44:10271-81 [Abstract]
    • Freeman, L, Lomas, A, Hodson, N, Sherratt, M, Mellody, K, Weiss, A, Shuttleworth, A, Kielty, C. Fibulin-5 interacts with fibrillin-1 molecules and microfibrils. Biochemical Journal. 2005; 388:1-5 [Abstract]
    • Mackay, J, Muiznieks, L, Toonkool, P, Weiss, A. The hydrophobic domain 26 of human tropoelastin is unstructured in solution. Journal of structural biology. 2005; 150:154-62 [Abstract]
    • Wise, S, Mithieux, S, Raftery, M, Weiss, A. Specificity in the coacervation of tropoelastin: solvent exposed lysines. Journal of structural biology. 2005; 149:273-81 [Abstract]
    • Mithieux, S, Wise, S, Raftery, M, Starcher, B, Weiss, A. A model two-component system for studying the architecture of elastin assembly in vitro. Journal of structural biology. 2005; 149:282-9 [Abstract]
    • Gheduzzi, D, Guerra, D, Bochicchio, B, Pepe, A, Tamburro, A, Quaglino, D, Mithieux, S, Weiss, A, Pasquali Ronchetti, I. Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers. Matrix biology: journal of the International Society for Matrix Biology. 2005; 24:15-25 [Abstract]
    • Mithieux, S, Weiss, A. Elastin. In: Advances in protein chemistry. United States: Academic Press 2005. p. 437-61. [Abstract]
    • Getie, M, Schmelzer, C, Weiss, A, Neubert, R. Complementary mass spectrometric techniques to achieve complete sequence coverage of recombinant human tropoelastin. Rapid Communications in Mass Spectrometry. 2005; 19:2989-2993 [Abstract]
    2004
    • Reddel, C, Weiss, A. Lamin A expression levels are unperturbed at the normal and mutant alleles but display partial splice site selection in Hutchinson-Gilford progeria syndrome. Journal of medical genetics. 2004. p. 715-717. [Abstract]
    • Clarke, A, Weiss, A. Microfibril-associated glycoprotein-1 binding to tropoelastin: multiple binding sites and the role of divalent cations. European journal of biochemistry / FEBS. 2004; 271:3085-3090 [Abstract]
    • Rock, M, Cain, S, Freeman, L, Morgan, A, Mellody, K, Marson, A, Shuttleworth, C, Weiss, A, Kielty, C. Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link. The Journal of biological chemistry. 2004; 279:23748-23758 [Abstract]
    • Rodgers, U, Weiss, A. Integrin alpha v beta 3 binds a unique non-RGD site near the C-terminus of human tropoelastin. Biochimie. 2004; 86:173-178 [Abstract]
    2001
    • Dedic, J, Weiss, A, Katahira, J, Yu, B, Trent, R, Urbán, Z. A novel elastin gene mutation (1281delC) in a family with supravalvular aortic stenosis: a mutation cluster within exon 20. Human mutation. 2001; 17:81 [Abstract]

    Publication Keywords

    Proteins; Elastin; Hydrogels; Tropoelastin; Dermis; Biochemistry; Biomedical Technology; Elastic Tissue